EFFECTIVENESS OF OVINE FOLLICULAR FLUID AND EWE AGE ON IN VITRO NUCLEAR MATURATION AND EMBRYO EVOLUTION OF AWASSI EWES

Two experiments were conducted to study the efficiency of ovine follicular fluid (OFF) and age ewe on in vitro nuclear maturation Awassi sheep oocytes (Experiment 1) subsequent stages early embryos their quality 2). The OFF has been added basic medium (TCM-199) with three different levels (A: 0% (Control group), B:15%, C:25% D:50%) while ages ewes determined into two main groups (> 2 1-2 year). In first experiment, across groups, matured C solution achieved highest (p=0.006) rates metaphase-II (85.70 82.35 % respectively). second belonging > 1-2-year (C Solution) (p=0.001) fertilization (23.17 30.73% Similar trend applies cleavage stage, reached 35.14 41.27% respectively (p=0.01). No significant differences noticed at (2-4 cell, morula, blastocyst). resulting B 2-year group) (1-2-year (p=0.04) Type1 (69.56 76.92% A difference was type2 (p=0.02), (Solution D) (46.15 33.33% It can be concluded that adding 25% media led improvement maturation, type1 oocytes.